# DNA Analysis Challenges Quebec Family Lore of Indigenous Ancestry Genetic testing of colonial Montreal remains reveals exclusively European ancestry in cemetery burials. - **Source:** [Earth.com](https://www.earth.com) - **Published:** 2026-11-12 - **Topic:** [science](https://brifing.si/c/science) - **Original article:** - **On Brifing:** https://brifing.si/s/07a2c7f4-f458-465d-a52f-b06c47d5fdcf ## Challenging Historical Narratives Many Quebec families maintain oral traditions of Indigenous ancestry, often citing the early colonial era. However, a new study analyzing the DNA of 45 individuals from Montreal’s Notre-Dame cemetery reveals exclusively European genetic markers. This research, published in PNAS, provides a scientific perspective on the demographic composition of early French colonial settlements. ## Methodology and Sample Selection Researchers examined remains from a cemetery used between 1683 and 1796. After excluding samples with modern contamination, the team successfully sequenced 45 genomes from petrous bones and teeth. These results were compared against 900 European, 751 West African, and 673 Indigenous American genomes to determine the precise ancestral origins of the buried individuals. ## Exclusively European Genetic Profiles All 45 individuals clustered genetically with Western European populations, specifically matching those from France. While two individuals possessed rare maternal lineages found in Indigenous or African populations, these markers are also present in European groups at low frequencies. The absence of mixed-ancestry genomes suggests these specific individuals were likely of European descent. ## Contextualizing Social Separation The lack of Indigenous or African ancestry in the cemetery does not prove such unions never occurred. Instead, researchers suggest it reflects the social and physical segregation of the colonial town. Indigenous populations were increasingly pushed away from urban centers, and colonial records confirm the presence of enslaved people within the region. ## Defining Indigeneity vs Ancestry Anthropologist Diane Martin-Moya emphasizes that genetic data should not be conflated with identity. She argues that Indigeneity is a political and cultural status rooted in kinship and community, rather than just blood ties. This distinction is crucial as claims of distant ancestry are sometimes used to pursue land-related legal rights. ## Family Structures and Diversity Genetic analysis identified five extended families within the cemetery, including uncle-nephew and grandparent-grandchild pairings. Despite the small size of the early colony, the population showed significant genetic diversity with no evidence of inbreeding. Relatives were often buried in different sectors, contradicting earlier archaeological theories that families were interred in single plots. ## Limitations of Archaeological Data The study acknowledges that these 45 individuals represent only a small fraction of the colonial population. The research was restricted to one specific churchyard, leaving the burial sites of Indigenous and African-descended residents unknown. Furthermore, the low-depth sequencing means that identified family trees remain hypotheses rather than definitive genealogical records. ## Future Research Directions Moving forward, researchers aim to move beyond bone analysis by utilizing sedimentary ancient DNA found in soil. The goal is to integrate genetic findings with archival records and oral traditions. This interdisciplinary approach seeks to tell a more inclusive history while respecting the perspectives of Indigenous communities regarding their ancestral remains. ## Sources covering this story - https://www.earth.com/science/family-lore-of-an-indigenous-ancestor-is-common-in-quebec-but-the-dna-from-the-first-french-colonists-suggests-otherwise/ — --- _Summary written by Brifing from the original reporting. Copyright in the original article belongs to its publisher; read the full piece at the link above._